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T Lymphocytes as Targets of Gene Transfer with Moloney-Type Retroviral Vectors

[ Vol. 1 , Issue. 4 ]

Author(s):

F. A. Ayuk, A. R. Zander and B. Fehse   Pages 325 - 337 ( 13 )

Abstract:


Peripheral T lymphocytes are a target of choice for many gene therapeutic strategies. Retrovirus-mediated transduction allows genomic integration and long-term expression of transgenes in target cells. Over many years, low transduction efficiency into primary T lymphocytes has limited clinical application of existing protocols. Recently, gene transfer rates > 50% have been achieved facilitating clinical studies. More attention is thus being focused on the ability of gene-modified cells to carry out innate as well as conferred functions in vivo and the influence of culture conditions, retroviral vector and host response thereon.

, T, Lymphocytes, as, Targets, of, Gene, Transfer, with, Moloney-Type, Retroviral, Vectors

Affiliation:

Bone Marrow Transplantation, University Hospital Eppendorf, Martinistr. 52, 20251 Hamburg, Germany.



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