Masoumeh Fardi*, Ali Mohammadi, Behzad Baradaran and Sahar Safaee
Introduction: Acute myeloid leukemia (AML) is the most prevalent type of adult hematopoietic system leukemia. Conventional therapies are associated with unfavorable side effects in individuals diagnosed with AML. These aftereffects with partial remission reflect the urgent need for novel therapeutic approaches for inducing apoptosis, specifically in malignant cells without affecting other cells. As a transcription factor (TF), ZEB2 (Zinc Finger E-Box Binding Homeobox 2), regulates the expression of specific genes in normal conditions. However, increased expression of ZEB2 is reported in various cancers, especially in AML, which is related to a higher degree of apoptosis inhibition of malignant cells. In this work, the role of ZEB2 in apoptosis inhibition is surveyed through ZEB2 specific knocking-down in human myeloid leukemia HL-60 cells.
Materials and Methods: Transfection of HL-60 cells was conducted using ZEB2-siRNA at concentrations of 20, 40, 60, and 80 pmol within 24, 48, and 72 h. After determining the optimum dose and time, flow cytometry was used to measure the apoptosis rate. The MTT assay was also applied to evaluate the cytotoxic impact of transfection on the cells. The expression of candidate genes was measured before and after transfection using qRT-PCR.
Results: According to obtained results, suppression of ZEB2 expression through siRNA was associated with the induction of apoptosis, increased pro-apoptotic, and decreased anti-apoptotic gene expression. Transfection of ZEB2-siRNA was also associated with reduced cell proliferation and viability.
Conclusion: Our study results suggest that ZEB2 suppression in myeloid leukemia cells through apoptosis induction could be a proper therapeutic method.
Acute myeloid leukemia (AML), ZEB2 (Zinc Finger E-Box Binding Homeobox 2), RNAi, Targeted therapy, Apoptosis.
Immunology Research Center, Tabriz University of Medical Sciences,, Department of Cancer and Inflammation Research, Institute of Molecular Medicine, University of Southern Denmark, Odense,, Immunology Research Center, Tabriz University of Medical Sciences,, Immunology Research Center, Tabriz University of Medical Sciences