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miR-375 Inhibits the Proliferation, Migration and Invasion of Esophageal Squamous Cell Carcinoma by Targeting XPR1

Author(s):

Wenbin Wu, Yangmei Zhang, Xiaowu Li, Xiang Wang and Yuan Yuan*   Pages 1 - 9 ( 9 )

Abstract:


Objective: The purpose of this study was to explore the mechanism of the miR-375/XPR1 axis in esophageal squamous cell carcinoma (ESCC) and provide a new idea for targeted therapy of ESCC.

Methods: Differentially expressed genes in GEO and TCGA databases were analyzed by bioinformatics. The expression levels of miR-375 and XPR1 mRNA were detected by qRT-PCR. Protein expression of XPR1 was detected by western blot. Bioinformatics analysis and dual luciferase assay were conducted to confirm the targeting relationship between miR-375 and XPR1. The viability, proliferation, migration and invasion of cells in each treatment group were detected by CCK-8, colony formation, wound healing and Transwell assays.

Results: Significantly down-regulated miR-375 and remarkably up-regulated XPR1 were observed in ESCC tissue and cells. Overexpression of miR-375 inhibited proliferation, invasion and migration of ESCC cells, and greatly reduced the promoting effect of XPR1 overexpression on cell proliferation, migration and invasion. Dual luciferase assay confirmed that miR-375 targeted and inhibited XPR1 expression in ESCC.

Conclusion: These results demonstrate the regulatory role of the miR-375/XPR1 axis in ESCC cells and provide a new potential target for the precise treatment of patients with ESCC.

Keywords:

XPR1, miR-375, esophageal squamous cell carcinoma, proliferation, migration, invasionXPR1, miR-375, esophageal squamous cell carcinoma, proliferation, migration, invasion

Affiliation:

Department of Thoracic Surgery, Xuzhou Central Hospital, Xuzhou Medical University, Xuzhou, 221009, Department of Medical Oncology, Xuzhou Central Hospital, Xuzhou Medical University, Xuzhou, 221009, Department of Medical Oncology, Xuzhou Central Hospital, Xuzhou Medical University, Xuzhou, 221009, Department of Medical Oncology, Xuzhou Central Hospital, Xuzhou Medical University, Xuzhou, 221009, Department of Medical Oncology, Xuzhou Central Hospital, Xuzhou Medical University, Xuzhou, 221009



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